Methods for identification and analysis of time-resolved fluorescence or reflection images on extensive three-dimensional surfaces

2014 
The present invention relates to a method for determination and evaluation of time-resolved fluorescence or reflection images on extensive three-dimensional surfaces which are scanned by means of pulsed radiation (pointwise optical scanning), for example. For tissue characterization and diagnosis in medicine or for the study of mechanically difficult to access technical objects (such as. in explosion-proof environment). The object of the invention to provide a method for the determination and evaluation of time-resolved fluorescence or reflection images indicate to extensive three-dimensional surfaces is achieved in that for the determination and evaluation of parameters of time-resolved fluorescence or reflection images on a three-dimensional surface (230) of objects wherein a pointwise , pulse-shaped scanning of the three-dimensional surface (230) by means of illumination and detection beam with different distances from the Beleuchtungsstrahlaustritts- and detection beam entry point (210) of the pulse-shaped illuminated points of the three-dimensional object (230) are present and an illuminated first object point (231) and an illuminated second object point (232) located in a different distance to the Beleuchtungsstrahlaustritts- and detection beam entry point (210) is, in the period of the excitation and detection beam (221) of the first Obj ektpunkts (231) and the illumination and detection beam (222) of the second object point (232) differ and takes place pixel by pixel modeling of the different running times, with different run times in the determination of fluorescence lifetime from the different distances of the illumination beam exit point or detection beam entry point and lighted between different surface points shown are taken into account in a model function.
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