[Chemical constituents of stems and leaves of Salvia yunnanensis and their anti-angiogeneic activities].

Present study was focused on the chemical constituents of the stems and leaves of Salvia yunnanensis C.H.Wright and their anti-angiogeneic activities.The compounds were isolated by column chromatography over silica gel and Sephadex LH-20,and other isolation techniques.Their structures were elucidated on the basis of spectral analysis and chemical evidences.Their anti-angiogeneic activities were evaluated by the chicken chorioallantoic membrane(CAM) neovascularisation model.Seven compounds were separated and identified as(+)-spathulenol(1),5,7,4′-trihydroxyflavanone(2),β-amyrin(3),3β-hydroxy-12-ursene(4),2α,3β-dihydroxyursa-12-en-28-oic acid(5),ursolic acid(6) and 3-oxo-12-ursen-28-oic acid(7).Compounds 1,2,5 and 6 were obtained from this plant for the first time.Compounds 5(an oleanane compound) and 6(an ursane compound) could inhibit angiogenesis significantly in a dose-dependent manner.