Isolation,Mapping and Application of An LTR Fragment for Dasypyrum Genome

Dasypyrum genus is an important source for improving the genetic variability of wheat(Triticum aestivum).However,the tetraploid species,D.breviaristatum,has not been reported to introduce into wheat.In this study,random amplified polymorphic DNA(RAPD) analysis was performed on Dasypyrum breviaristatum and other 9 Triticeae materials by 142 random 10-based primers.A(1 182) bp Dasypyrum specific RAPD fragment was obtained,which was named OPH12_(1182).Sequence similarity analysis revealed that 95% of OPH12_(1182) was similar to a retrotransposon repeat sequence Sabrina of Hordeum vulgare,indicating that OPH12_(1182) was also a kind of retrotransposon repeat sequence.The result of fluorescence in situ hybridization(FISH) suggested that pDbH12 was specifically hybridized throughout all D.breviaristatum chromosomes arms except for the terminal and centromeric regions,which indicated that OPH12_(1182) is a specific repetitive sequence in genome of Dasypyrum.Furthermore,based on the sequence of OPM2_(1182),pairs of primers were designed,and used to amplify materials used in this study,the result showed that all the seven pairs of Dasypyrum chromosomes contained the specific DNA segment and OPH12_(1182) could be used to detect offsprings containing Dasypyrum chromatins and wheat-Dasypyrum amphiploids.Therefore,OPM2_(1182) could be used as a molecular marker for detection of chromatins of Dasypyrum.