The influence of vinegars on exposure of dentinal tubules: a SEM evaluation Influência de vinagres na exposição dos túbulos dentinários: avaliação em MEV

2004 
Dentin hypersensitivity is a common painful condition observed in clinics. Dietary habits have been much associated with its development and persistence during and following periodontal treatment. The aim of this in vitro study was to evaluate the influence of vinegars on the removal of smear layer and exposure of dentinal tubules. Extracted human teeth were submitted to manual scaling with Gracey curettes in order to remove the cementum as well as to form a smear layer. Dentin samples with 3 mm 2 were obtained and distributed into six experimental groups: one control and five types of vinegars (alcohol, apple, rice, white wine and balsamic). Each group included two methods of vinegar application: topical and friction. After routine preparation for SEM analysis, photomicrographs were assessed by a calibrated and blind examiner using an appropriate index system. Kruskal- Wallis test indicated a significant influence of vinegars on smear layer removal. There was a statistically significant difference between groups treated with apple, white and rice vinegars and the control group (p < 0.05). Neverthe- less, Mann-Whitney test indicated that removal of smear layer did not vary with the method of application (topical versus friction) for any of the tested substances. We can conclude that the contact of vinegar may remove smear layer and expose dentinal tubules, regardless of the type of application. However, balsamic vinegar was associated with less removal of smear layer after both methods of application. DESCRIPTORS: Dentin sensitivity; Diet; Smear layer. RESUMO: A hipersensibilidade dentinaria cervical e uma condicao dolorosa muito comum nos consultorios. A dieta tem sido bastante associada ao seu aparecimento, assim como a sua persistencia apos o tratamento periodontal. O objetivo deste trabalho foi avaliar in vitro a influencia dos vinagres na remocao de "smear layer" e exposicao dos tubulos dentinarios. Dentes de humanos foram instrumentados com curetas Gracey para a remocao do cemento e formacao de "smear layer". Foram obtidas amostras de dentina com 3 mm 2 , divididas entre o grupo controle (agua destilada) e cinco grupos de vinagre: branco, maca, arroz, vinho branco e balsâmico. Cada grupo incluiu duas formas de aplicacao da substância, topica ou por friccao. Apos o preparo para observacao em MEV (microscopia eletronica de varredura), as fotomicrografias foram avaliadas por um examinador previamente calibrado utilizan- do um indice apropriado. O teste de Kruskal-Wallis indicou influencia significativa dos vinagres na remocao de "smear layer". Foi constatada uma diferenca estatistica significante entre os grupos maca, branco e arroz e o grupo controle (p < 0,05). O teste de Mann-Whitney, porem, indicou que a remocao de "smear layer" nao variou para ne- nhuma das substâncias segundo a forma de aplicacao. Conclui-se que os vinagres podem remover "smear layer" da superficie radicular e expor tubulos dentinarios, nao sendo influenciados pelo tipo de aplicacao. Dentre os tipos de vinagres testados, o balsâmico esteve associado a menor remocao de "smear layer" apos ambas as formas de aplicacao. DESCRITORES: Sensibilidade da dentina; Dieta; Camada de esfregaco.
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