PI3K/AKT pathway mediates the anti-oxidative injury induced by H_2O_2 preconditioning in PC12 cell

Objective To explore whether PI3K/AKT pathway plays a role in H2O2 preconditioning-induced adaptive cytoprotection.Methods Setting up an experimental model of H2O2 preconditioning after injury induced by H2O2 at high concentration in cultured PC12 cells.The viability of cells was measured by MTT assay.The activity of lactate dehydrogenase(LDH) was assessed by colorimetry.The percentage of apoptotic cells was measured by flow cytometry(FCM) with propidium iodide stain.AKT were detected by Western blot.Results Preconditioning of H2O2 at 100 μmol/L for 90 min significantly protected PC 12 cells from 300 μmol/L H2O2-induced injury,increasing the cell viability from 50.2%±4.6% to 83.8%±3.5%,reducing the activity of LDH from 103%± 10.21% to 68.5%±5.34%,and decreasing the rate of apoptotic cells from 65.5%±4.1% to 37.1%±2.3%(P0.01).H2O2 preconditioning induced expression of p-AKT,and ly294002(an inhibitor of PI3K) blocked expression of p-AKT induced by H2O2 preconditioning.ly294002 markedly reduced the anti-cytotoxicity and anti-apoptosis induce by H2O2 preconditioning.Conclusions H2O2 preconditioning induced activation of AKT via PI3K-dependent pathway and activation of PI3K/AKT cascade mediated the adaptive cytoprotection induced by H2O2 precondition.