Single-turnover and multiple-turnover measurement of phytoplankton photosynthesis parameters using variable light pulse induced fluorescence

Using a measurement system based on fluorescence induced by variable pulse light, photosynthesis parameters of chlorella pyrenoidosa are obtained, employing single-turnover and multiple-turnover protocols under dark-adapted and light-adapted conditions. Under the light-adapted condition, σPSII′ is larger, and Fv′/Fm(ST)′ and Fv′/Fm(MT)′ are smaller than those of the dark-adapted condition, but the corresponding parameters possess good linear correlations. Fm(MT), Fm(MT)′, Fv/Fm(MT), and Fv′/Fm(MT)′, which are measured using the multiple-turnover protocol, are larger than those of the single-turnover protocol. The linear correlation coefficient between Fm(ST) and Fm(MT) is 0.984, and Fv/Fm(MT)=1.18Fv/Fm(ST). The linear correlation coefficient between Fm(ST)′ and Fm(MT)′ is 0.995, and Fv′/Fm(MT)′=1.36Fv′/Fm(ST)′.