The Effect of a Hemostatic Agent (FastAct) to Wound and Tissue Repair in a Rat Model

Objectives: The purpose of this study was to test the hypothesis that the hemostatic agent (FastAct) exerts favourable effect on wound healing and tissue repair alone and along with prilocaine hydrochloride in vivo. Wound healing process is a complex procedure that starts with bleeding and hemostasis. Comprising of bovine factor proteins II, VII, IX and X, FastAct is used as a catalyst in the clot formation process to activate factors II, V, VIII and XIII. Materials and Methods: The study was performed on 21 Wistar male rats. Cutaneous incisions at the length of 4 cm. were made on the dorsal side of all animals. In seven, FastAct was applied alone between the wound edges and, in the other seven, FastAct was applied along with prilocaine. The wounds were sutured by resorbable suture material. Results: The specimens were obtained on the seventh day. Wound breaking strength test was performed before histological examination. In the FastAct group the epithelialization and the closure of the wound edge were better than the other groups. The results of the breaking strength test showed that the results were best in FastAct group. Conclusion: Ita#39;s concluded that FastAct accomplished hemostasis, facilitated wound healing in accordance with natural processes. Amac: Bu calismanin amaci, in vivo olarak hemostatik bir ajanin (FastAct) prilokain hidroklorid ile beraber veya onsuz, yara ve doku iyilesmesini kolaylastirdigi hipotezinin test edilmesidir. Yara iyilesmesi kanama ve hemostaz ile baslayan kompleks bir prosedurdur. Sigir protein faktorleri II, VII, IX ve Xa#39;u ihtiva eden FastAct pihti olusumunda faktor II, V, VIII ve XIIIa#39;u aktive etmesi icin katalizor olarak kullanilir. Gerecler ve Yontemler: Calisma 21 erkek Wistar rat uzerinde gerceklestirildi. Tum hayvanlarin sirtinda 4 cma#39;lik kutanoz insizyonlar yapildi. Yedi hayvanda yara kenarlari arasina sadece FastAct uygulanirken, diger yedisinde FastAct prilokain ile birlikte uygulandi. Yara dudaklari rezorbe olan sutur materyali ile kapatildi. Bulgular: Ornekler yedinci gun elde edildi. Histolojik inceleme oncesinde orneklere germe ve cekme testleri uygulandi. FastAct grubunda epitelizasyon ve yara iyilesmesinin diger gruba gore daha iyi oldugu tespit edildi. Germe ve cekme testlerinde FastAct grubu sonuclarinin daha iyi oldugu gozlendi. Sonuc: FastActa#39;in hemostazisi sagladigi, dogal surecler yardimiyla yara iyilesmesini kolaylastirdigi sonucuna ulasilmistir.