Preparation of a polyvalent antiserum specific for human muscle proteins. II. Animal immunization and purification of the antiserum
1980
: A new preparation technique to raise rabbit antisera against human water-soluble muscle proteins is presented. Immunization, blood collection and further purification of antibody fraction in serum are described in detail. The present method is fast and easy to perform; chromatography and dialysis are not required and antisera so produced lend themselves to immunoelectrophoretic evaluation of human water-soluble muscle proteins.
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