IV type collagenase content measuring method

The invention discloses an IV type collagenase content measuring method, which relates to a method for measuring the content of IV type collagenase in blood plasma and blood serum of human bodies. The method is characterized in that the IV type collagenase has the gelatin hydrolyzing specific binding site, other matrix metalloproteinase existing in the blood plasma is difficult to hydrolyze the gelatin, TNBS is adopted for developing the color of gelatin hydrolysates, and the quantitative analysis on the specificity of the IV type collagenase in the living blood serum of the blood plasma of the human bodies can be carried out. The invention can accurately and fast measure the IV type collagenase content in the blood plasma or the blood serum of the human bodies.