Sex-dependent and organ-specific toxicity of silver nanoparticles in livers and intestines of adult zebrafish

Abstract Silver nanoparticles (AgNPs) have been increasingly manufactured and thus are increasingly detected in aquatic systems. However, there are still some overlooked factors (e.g., organism sex) in the field of nano-toxicological assessment. In this study, to explore the role of sex in nanotoxicity, adult male and female zebrafish were exposed to 100 μg/L of two uncoated commercial AgNPs with primary sizes 20 nm and 80 nm for 2 weeks, after which the impacts of AgNPs on intestines and livers of both male and female zebrafish were assessed using a suite of biomarkers. Results demonstrated that the intestinal Na/K-ATPase activity as well as the superoxide dismutase activity in male zebrafish differed significantly between 20-nm AgNPs and 80-nm AgNPs treatments (p