RNA-Live-Imaging in Mikroorganismen

RNA live imaging is a powerful technique to visualize the subcellular localization as well as highly dynamic transport of mRNAs in vivo. Established in eukaryotic microorganisms, it is now a wide-spread system used in bacteria, animals, and plants. The method is based on the binding of heterologous RNA-binding proteins to their cognate binding sites that are integrated into the mRNA of interest. Recent advances enable the visualization of transcriptional or translational sites as well as mRNA decay.