Detecting and bioimaging of hypochlorite by a conjugated fluorescent chemosensor based on thioamide

Abstract A novel thioamide-based conjugated probe SNC, (E)-2-((E)-3-(4-(dimethylamino)phenyl)allylidene)-N,N-dimethylhydrazine-1-carbothioamide, has been designed. Probe SNC could selectively recognize ClO- in aqueous media through fluorescence turn-off and sensitively detect ClO- down to 0.90 μM. In addition, successful detection of hypochlorite was achieved for practical applications in living zebrafish and water samples, and the detection limit turned out to be 2.26 μM. Detection process between SNC and hypochlorite was illustrated through UV–Vis spectroscopy, 1H NMR titration, ESI-mass and DFT study.