Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi

Abstract High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the c lpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. “Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp.” [1].