分子信标研究5-氟尿嘧啶对鼻咽癌HNE1细胞p33 ING1 基因转录水平的影响

In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells, p33(superscript ING1) mRNA level in cellular total RNA was detected quantitatively based on MB assay. During the operation, the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33(superscript ING1) were measured in vitro. The results were as follows: p33(superscript ING1) mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time. The MTT results also proved that high expression of p33(superscript ING1) mRNA can increase cell's sensitivity to chemical drug 5-FU. The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.