LC-MS/MS Estimation of Propranolol level in Exhaled Breath Condensate
2017
Background: Exhaled breath condensate (EBC) could be used
as a non-invasive and alternative specimen to urine and blood for monitoring
propranolol levels. A simple, sensitive and selective liquid
chromatography–tandem mass spectrometry (LC–MS/MS) method is employed for the
determination of propranolol in EBC samples.
Methods: Samples
directly injected to a C18 analytical column and isocratically
separated using a mobile phase composed of methanol + acetic acid (99:1 v/v).
Detection was performed by positive electrospray ionization in multiple
reaction monitoring and selected ion recording modes.
Results: The
chromatographic separation was obtained within 6.0 min and was linear over the
concentration range of 5.6–224.0 ng/mL (R2 = 0.999). The accuracy
and precision of the method were within 15% according to FDA guideline. The
found concentrations of propranolol in EBC of two patients receiving 80 mg/day
were 30 and 40 ng/mL.
Conclusion: Developed
method was applied to determine propranolol levels in three patients receiving
propranolol in their medication. The obtained propranolol levels in EBC could
be used to develop simpler, cheaper and more feasible analytical methods to be
used in routine analysis of propranolol in biomedical analytical laboratories.
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