Fish and mammalian liver cytosolic glutathione S-transferases: Substrate specificities and immunological comparison

Abstract The substrate specificities of cytosolic glutathione S-transferase (GST) activity were compared in several common marine fish, anadromous and freshwater salmonids. Polyclonal antisera raised against purified rat and plaice GST subunits were used to investigate phylogenetic relationships between the mammalian and fish enzymes. Flatfish contained two major isoforms, one of which is related to the mammalian group 1 family. The major GST of salmonids and the cod was related to the mammalian group 3 transferase used as a preneoplastic marker.