Identification in Immuno-Electron Microscopy of Solitary Multi-Polar Peripheral Neurons of Adult Opisthorchis viverrini by Antibody Against Rat Diacylglycerol Kinaseζ

By utilizing the antibody for rat DGKz a substantial number of immunopositive cells were found in the OV (Opisthorchis viverrini). The immunopositive cells appeared solitarily and they were distributed rather symmetrically to the longitudinal axis of the OV. Some of them were located in close proximity to internal organs such as uterus, ovary, testes, vitelline glands and guts. The immunostained cells extended tapering processes horizontally or obliquely to the OV longitudinal axis. In immuno-electron microscopy, the immunopositive cells were characterized by intensely immunostained mitochondria and weakly immunostained cytoplasm and immunonegative chromatin-poor nucleus. Vacuoles of various sizes without the immunoreactivity were also contained in the cells. Thin cellular processes without the immunoreactivity were found to enclose thinly the entire surfaces of the immunostained cells and processes, and they were in continuity with the interstitial partition-like processes which contained nuclei and aggregation of microfibrils at some distance from the cytoplasmic envelopes. The present finding suggests the possibility that the immunostained cells were peripheral neurons enveloped by peripheral glia and that the glia are of mesenchymal origin because of their cytoplasmic continuity to the interstitial partition-like processes. The motor or sensory nature of the neurons remains to be elucidated. Mediante el uso del anticuerpos DGK para rata se determino un numero considerable de celulas inmunopositivas en el Opisthorchis viverrini (OV). Las celulas inmunopositivas aparecian solitarias y se distribuian simetricamente al eje longitudinal de la OV. Algunas estaban ubicadas en las proximidades de los organos internos como el utero, ovarios, testiculos, glandulas vitelinas e intestino. Las celulas inmunotenidas extendian sus procesos horizontalmente u oblicuamente al eje longitudinal de la OV. Por microscopia inmunoelectronica, las celulas inmunopositivas se caracterizaron por presentar mitocondrias intensamente tenidas, citoplasma con tincion debil e inmunonegatividad en nucleos pobres en cromatina. Tambien se observo en las celulas, vacuolas de diversos tamanos sin inmunorreactividad. Se encontraron procesos celulares sin inmunorreactividad para cerrar finamente todas las superficies de las celulas y procesos, y se continuaron con los procesos de particion intersticiales que contenian nucleos y agregacion de microfibrillas a cierta distancia de las envolturas citoplasmicas. El presente hallazgo sugiere la posibilidad de que las celulas inmunotenidas son neuronas perifericas envueltas por glia periferica y que la glia presenta origen mesenquimal debido a su continuidad citoplasmatica con los procesos de particion intersticiales. La naturaleza motora o sensorial de las neuronas aun no se ha dilucidado.