Method for recycling active ingredients in amoxicillin mother liquor synthesized by enzymatic method

The invention discloses a method for recycling active ingredients in the amoxicillin mother liquor synthesized by an enzymatic method. The method includes synthesizing the amoxicillin mother liquor by the enzymatic method; separating the amoxicillin mother liquor through a macro-porous resin column, eluting the separated amoxicillin mother liquor by deionized water, and collecting an eluant rich in D-4-Hydroxyphenylglycine and an eluant rich in 6-amino penicillanic acid (APA) respectively; filtering the eluant rich in D-4-Hydroxyphenylglycine with Daltonian ultrafiltration membranes with a cutoff molecular weight of 150 to 200; performing nanofiltration concentration on the Daltonian ultrafiltration membranes for the filtered liquor with the cutoff molecular weight of 150 to 200, and standing, crystallizing and filtering the concentrated liquor to obtain solids, and drying the solids to obtain the D-4-Hydroxyphenylglycine. According to the method, the technological design is reasonable, the operation is convenient, the recycling effect is good, and energy is saved and the environment is protected.