Selectivity Encapsulation of Zingiber cassumunar Roxb. oil by Beta-cyclodextrin Derivatives

Abstract Zingiber cassumunar Roxb. (Plai) is a medical plant widely cultivated in Asia and its essential oil has been reported for several biological activities, such as anti-inflammatory, anti-microbial and anti-oxidant effects. In this work, the essential oil was extracted from Plai by steam distillation with 0.32% yield. (E)-1-(3,4-dimethoxyphenyl) butadiene (DMPBD), (E)-1-(3,4-dimethoxyphenyl) but-3-ene-1-ol (compound D) and terpinen-4-ol were reported as the major components. The components of Plai oil were investigated by GC-MS. It was found that sabinene, terpinen-4-ol and DMPBD were detected as the major components for 23, 20 and 30%, respectively. The three types of beta-cyclodextrins (bCDs) i.e. beta-cyclodextrin (bCD), 2-hydroxypropyl-beta-cyclodextrin (HPbCD) and citric acid crosslinked beta-cyclodextrin (pbCD) were used to form the inclusion complexes with Plai oil to increase the aqueous solubility, selectivity and anti-analgesic properties. The ratio of CDs and plai oil was varied. The amount of terpinen-4-ol and DMPBD were detected by GC-MS. The results showed that terpinen-4-ol was fitted to bCD whereas HPbCD showed the highest selectivity of DMPBD. The pbCD presented the lowest selectivity both terpinen-4-ol and DMPBD. As the results, the different types of CDs showed the different selectivity for inclusion complex formation with major component in Plai oil. The separation of terpinen-4-ol and DMPBD can be done by using CD inclusion complexes. The anti-analgesic property of Plai oil and its complexes will be further studied.