Two novel linkage SNPs of VLDLR gene intron 11 are associated with laying traits in two quail populations

Abstract. The very low density lipoprotein receptor (VLDLR) is an important multifunctional receptor and plays a key role in chicken reproduction. This study is designed to investigate the effect of variants in the VLDLR gene on quail laying traits. Two quail populations were studied – yellow feather quail and chestnut feather quail; 384 individuals per breed were used. The laying traits (the weight of the first egg, the age of the first egg, egg weight, the weight of 20-week-old and the egg number of 20-week-old) were measured and recorded. The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was developed to genotype those individuals. The results showed two novel polymorphisms, i.e. two linkage variations in intron 11 of the VLDLR gene (363T > C and 392C > T). They are associated with the weight of the first egg, the age of the first egg and egg number of 20-week-old in yellow feather and chestnut feather quail. The two variations in intron 11 of VLDLR may be linked with potential major loci or genes affecting some laying traits.