Lipidomic-based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD.

Background In recent years, with the improvement of people's living standards, non-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease in the world. Methods In this paper, the metabolic disorders in SD rats were induced by choline-deficient, L-amino acid-defined (CDAA) diet. The therapeutic effects of polyene phosphatidylcholine (PPC) and Babaodan (BBD), on NAFLD were observed. The lipidomic analysis was performed using UPLC-Orbitrap MS, and data analysis and lipid identification were performed using the software Lipidsearch. Results Both PPC and BBD can reduce lipid accumulation in the liver and improve abnormal biochemical indicators in rats, including reduction of triglycerides (TG), total cholesterol (TC), ALT and AST in serum. In addition, lipids in rat serum were systematically analyzed by means of lipidomics in this study. The lipidomic results showed that the most obvious lipids with abnormal metabolism in CDAA diet-induced rats were glycerides (TG and DG), phospholipids and cholesterol esters. Conclusion Both BBD and PPC partly reversed the lipids disturbance induced by the CDAA diet. PPC may be more effective than BBD in alleviating NAFLD because it has a better effect on inhibiting the abnormal accumulation of lipids and reducing the inflammatory reaction in the body.