TO DETECT THE SUDAN I IN FOOD USING METHOD OF RE-HPLC

Objective:To evaluate the method of detection of Sudan I in foods based on the use of RE-HPLC.Methods:Sudan 1 in foods were detected by re-HPLC-DAD after extracted by alcohol.Results:This method could detect Sudan I in samples effectively while its concentration varied from 0.5 μg/ml to 1 000 μg/ml.The standard deviation was 0.18,relative standard deviation was 0.73%,and average recovery was 94.43%.0.2 ng was observed as its detection limit,while the concentration limit was 0.2 mg/Kg.Artificial synthesized pigments had no disturbance in the process.Conclusion:This method is validated in terms of detection limits,linearity,sensitivity,accuracy and selectivity in detection of Sudan I in foods.