Determination of six type A and type B trichothecenes by high performance liquid chromatography-tandem mass spectrometry.

Objective To establish a method for the simultaneous determination of six type A and type B trichothecenes by high performance liquid chromatography-tandem mass spectrometry. Method The homogenized sample was extracted by 84% acetonitrile-water solution (V/V) and further purified by MycoSep 226 or MycoSep 227. The target compounds were separated by waters Atlantis dC18column and detected by MS/MS system with eletrospray ionization (ESI) under multiple reaction monitoring (MRM) mode. Results It was shown that the high correlation coefficients (r≥0.996) of six mycotoxins were obtained within their respective linear ranges. The LOQ for the six compounds ranged from 0.1~20 μg/kg. The mean recoveries ranged from 60.1%~123% with the relative standard deviations of 7.5%~19.8%. Conclusion This method was suitable for the identification and quantification of multi-component mycotoxin contaminants in complex matrixes with advantages of simple pretreatment, good purification and high sensitivity.