Evaluación del potencial genotóxico de Boerhavia erecta L

Se evaluo la actividad mutagenica de un extracto acuoso de follaje liofilizado de Boerhavia erecta L. Se emplearon 2 pruebas: de Ames e induccion de micronucleos en medula osea de raton. En el ensayo de Ames, los resultados fueron negativos en todas las cepas probadas de Salmonella typhimurium: TA 1535, TA 1537, TA 98 y TA 100, con un protocolo de incorporacion en placa y concentraciones hasta 5 mg de liofilizado/placa. En el ensayo de micronucleos, se hicieron 2 administraciones orales a razon de 10 mL/kg, separadas 24 h, con sacrificio 24 h despues de la ultima aplicacion. Las dosis fueron 500, 1 000 y 2 000 mg de liofilizado/kg. No se observaron variaciones en la relacion entre eritrocitos policromaticos y eritrocitos normocromaticos, indicador de toxicidad medular. Tampoco se encontraron alteraciones significativas en la frecuencia de eritrocitos policromaticos micronucleados entre los distintos grupos de tratamiento. Se comprobo que el extracto acuoso de follaje liofilizado de B erecta no parece inducir efectos mutagenicos en los 2 sistemas de ensayo donde se evaluo The mutagenic activity of an aqueous extract of freeze-dried foliage of Boerhavia erecta L. was evaluated. 2 tests were used: Ames' test and micronucleus induction in mouse bone marrow. In Ames' test, the results were negative in all the tested strains of Salmonella typhimurium : TA 1535, TA 1537, TA 98 and TA 100, with a protocol of incorporation to plaque and concentrations up to 5 mg of freeze-dried/plaque. In the micronucleus test, 2 oral doses of 10 mL/kg were administrated separated by 24 hours, with sacrifice 24 hours after the last application. The doses were 500, 1 000 and 2 000 mg of freeze-dried/kg. No variations were observed in the relation between polychromatic erythrocytes and normochromatic erythrocytes, an indicator of medullary toxicity. No significant alterations were observed in the frequency of the micronucleated polychromatic erythrocytes among the different treatment groups. It was proved that the aqueous extract of freeze-dried foliage of B. Erecta does not seem to induce mutagenic effects in the two assay systems where it was assessed.