Transcriptional profiling of cells sorted by RNA abundance

Sandy L. Klemm,Stefan Semrau,Kay Wiebrands,Dylan Mooijman,Dina A. Faddah,Rudolf Jaenisch,Alexander van Oudenaarden

Transcriptional profiling of cells sorted by RNA abundance

2014

Sandy L. Klemm
Stefan Semrau
Kay Wiebrands
Dylan Mooijman
Dina A. Faddah
Rudolf Jaenisch
Alexander van Oudenaarden

Methods are described for preparing unbiased libraries for transcriptome profiling of cells sorted according to the abundance of a transcript of interest.

Keywords:

Induced pluripotent stem cell
Gene expression
Gene expression profiling
Fluorescence in situ hybridization
RNA
Transcriptome
Molecular biology
Biology
Genetics
Bioinformatics
Cell culture
Embryonic stem cell
RNA extraction
Reprogramming

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