The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse

The vascularization and the dermal remodeling of two different types of human skin reconstructed «in vitro» and grafted onto the nude mouse were studied. They were composed of human keratinocytes grown either on a human acellular deepidermized dermis (DED), or on a lattice composed of human fibroblasts embedded in bovine type I collagen, a living dermal equivalent (LDE). At different stages alter grafting, the transplants were harvested and proceased for an immunohistological study with species-specific and non-species-specific antibodies