Effect of dimethyl sulfoxide on cytosolic calcium in cultured rat hepatocytes injured by D-galactosamine

D-galactosamine (Gal 0.5 mmol.L-1) made lactate dehydrogenase (LDH) leakage from the hepatocytes in monolayer-culture increase by 50%, Dimethyl sulfoxide (Me2SO 2% vol/vol) decreased the LDH leakage (P less than 0.05). The cytosolic free Ca2+ concentration ([Ca2+]c) of rat hepatocytes exposed to Gal 4 mmol.L-1 for 90 min in suspension culture increased about 2-fold (P less than 0.01). Me2SO (2%) antagonized this [Ca2+]c-increasing effect of Gal. These results verified directly that the [Ca2+]c of hepatocytes was increased in the early stage of Gal-induced hepatotoxicity, and suggested that the prevention or lightening of the disturbance in intracellular Ca2+ homeostasis may be, at least, one of the mechanisms of the protective action of Me2SO against Gal-induced hepatocyte injury.