Screening and identification of Actinomycetes produced chitinolytic from suppression soil as biological agents of fruit flies ( Bactrocera sp.)

The effectiveness of biological control is generally associated with the tolerance of biological control agents to environmental stress. One of the enhanced tolerances of entomopathogenic bacteria to thermal factors and pesticides is a result of the chitinolytic biosynthesis of the filamentous bacteria. The filamentous bacteria Actinomycetes can be isolated from several specific areas: the Kelud volcano mountain, a tomato field contaminated with pesticides and protected forests. The aim of the study was to obtain and identify Actinomycetes candidates capable of producing chitinolytic compounds and controlling fruit flies and other insects (Bactrocera sp.) in vitro. Screening and identification were undertaken using descriptive methods while an antagonism test was designed using a completely randomized block design, with nine treatment combinations and each treatment being replicated three times. The results showed that Actinomycetes Tomatoes_Pare (Atp) and Actinomycetes Merubetiri 1 and 2 (Mbr1 and Mbr2) isolates produce chitinase and are able to control parasite fruit fly larvae and pupae by up to 100% in vitro. The identification of Actinomycetes spp. by DNA sequencing showed that 3 Actinomycetes isolates have are closely related: Streptomyces narbonesis, Streptomyces spectabilis SHH and Streptomyces deserti.