Improved method of using scanning electron microscopy to study the morphology of Isodidae family

Preparation method for scanning electron microscope is a fundamental process in achieving an excellent result in getting ultrastructure micrograph of a house dust mite sample. The objectives of this study are therefore to determine a simpler method of preparing the sample for a better view and excellent images using SEM and to compare the outcome images of its morphological characters based on the new preparation method of which glutaraldehyde and osmium tetra-oxide technique in fixation was replaced with the use of acetone. This modified method gives improved images in SEM and it is very facile as it reduces the processing time by 65 % when compared to the glutaraldehyde and osmium tetra-oxide method.