Effects of the Agglutinins Extracted From Rhizoctonia solani (Cantharellales: Ceratobasidiaceae) on Pieris brassicae (Lepidoptera: Pieridae)

Lectins are widespread proteins found in plants, fungi, bacteria, and vertebrates, and they play the critical roles in many physiological functions. Two lectin molecules (namely, RSAI and RSAII) were extracted from Rhizoctonia solani Kuhn and their effects on Pieris brassicae L. larvae were determined by larval survival rate, body mass, nutritional indices, digestive enzyme activities, and caspase-3 gene expression. The highest mortality caused by RSA treatment was recorded up to 80%, the larval weight decreased to 0.05 g and Similarly, RSAs significantly decreased nutritional indices including conversion efficiency of ingested food (ECI), conversion efficiency of digested food (ECD), approximate digestibility (AD), relative consumption rate (RCR), and relative growth rate (RGR) in a dose-dependent manner. Activities of α-amylase and α- and β-glucosidases significantly decreased in the larvae fed with RSA-treated diets. Also, activities of TAG-lipase and proteases significantly reduced after feeding with different concentrations of RSAs. Gene expression analysis of caspase-3 in control and treated larvae revealed significant increment of its expression in the larvae fed with RSAI and RSAII, respectively, 9.52- and 1.47-fold compared to control. These results clearly demonstrated insecticidal effects of R. solani lectins on P. brassicae via several physiological pathways, thus rendering RSA as a good target for furthering our knowledge and suggesting new strategies to overcome pesticide side effects.