A method of detect the virus which caused diarrhea

Objective Building a method which can examines virus pathogenic in gnstroenteritis excrement specimen.Methods Choosing six positive specimens which tested in our laboratory,include adenovirus,calicivirus,rotavirus,bocavirus,astrovirus and enterovirus.Through sequence-independent single primer amplification(SISPA) constructs a gene bank.Looks up the viral gene fragment in gene bank.Results Obtaining corresponding viral acid sequence in six specimens.Conclusion This research can examine enterovirns and the virus which cause diarrhea,It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present. Key words: Gastroenteritis; Noxae; Sequence-independent single primer amplification