A simple determination for 8 kinds of organophosphorus pesticides from animal tissues. Application to toxicological experiments.

A simple determination for eight kinds of organophosphorus pesticides (OPs) from animal tissues was newly developed. OP was extracted from the sample with acetonitrile, added 2% NaCl solution, and reextracted with n-hexane. The organic layer was loaded to a Sep-Pak silica cartridge and OP retained in the cartridge was eluted with ethyl acetate. The eluate was dried up under a stream of nitrogen. The residue was dissolved in n-hexane and the OP was determined by use of gas chromatograph (GC) equipped with a flame photometric detector (FPD). When nine OPs were separately added to four tissues (muscle, liver, adipose tissue and blood) of hen at the level of 5μg per 0.5 g of tissue weight, recoveries of fenitrothion, malathion, fenthion, parathion, cyanofenphos, EPN and leptophos ranged from 79.8% to 96.9%. In the case of cyanophos, recoveries from muscle, liver and adipose tissue were from 83.1% to 91.9%, but recovery from blood was 60.1%. Recoveries of dichlorvos from muscle, liver, adipose tissue and blood were below 18.8%. These results demonstrate that this simplified method is applicable to the determination of the above eight OPs except dichlorvos from animal tissues.