Sequence Characterization and Differential Expression of a Hemolysin Gene and the Encoded Protein from Sparassis latifolia

The mRNA sequence of an aegerolysin-like gene from Sparassis latifolia,namely latifolysin,was obtained by transcriptome sequencing and analyzed using bioinformatics-based methods.Latifolysin contained an ORF of 399 bp encoding 133 amino acids,and the deduced latifolysin protein incorporated domains characteristic of the aegerolysin family,exhibiting 40％ identity to aegerolysin.Phylogenetic analysis revealed latifolysin to be clustered within a group of basidiomycetes,and closely related to an aegerolysin-like protein from Volvariella volvacea.Expression levels of latifolysin and latifolysin were determined using quantitative real-time PCR and isobaric tags for relative and absolute quantification (iTRAQ) coupled with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) respectively,and shown to be highest at the young fruit body stage.