Studies on Effects of t10,c12-CLA on Lipid Metabolism of Subcutaneous and LongissimusMuscle Tissues in Pig

In vitro tissue culture system of subcutaneous(SC)adipose and longissimus muscle taken from 30 d pig were constructed firstly,0.1 mmol/L bovine serum albumin(BSA,as control),100 μmol/L t10,c12-CLA were added to the cultures for 10 d,six replicates were performed for each treatment.At the end of 10 d treatment period,cells were harvested and stored at-70 ℃.Real-time RT-PCR and kit assay were used to characterize the effects of t10,c12-CLA on the lipogenic and lipolytic enzymes,regulation hormones,TG content in longissimus muscle and SC adipose of pig.The results showed that:the mRNA abundance of FAS and INSR,TG content in SC adipose tissue and the mRNA abundance of HSL and LPL in longissimus muscle tissue were decreased by adding t10,c12-CLA(P0.05);by contrast,HSL mRNA abundance in SC adipose tissue and the mRNA abundance of INSR,TG content in longissimus muscle tissue were increased by adding t10,c12-CLA(P0.05).These results illustrated that the effects of t10,c12-CLA on lipid metabolism and deposition of SC adipose tissue differed from that on longissimus muscle tissue,and further confirmed that t10,c12-CLA inhibited the lipid deposition of SC adipose and promoted the lipid deposition of longissimus muscle in pig.