Adventitious shoot regeneration from root tips of intact seedlings of Aegle marmelos

SummaryIntact seedlings of Aegle marmelos Corr. were tested for their ability to produce adventitious shoots by direct culture of mature seeds on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyladenine (BA); 1–2 mg l–1 BA was found to be the optimum concentration. Addition of 0.1 mg l–1 indoleacetic acid (IAA) further increased shoot proliferation efficiency. Shoot buds originated from regions adjacent to the bases of cotyledons and cotyledonary axils and roots. In many cases, adventitious shoots were produced from the enlarged apical region of root. Shoots were rooted on MS medium supplemented with indolebutyric acid (IBA). Rooted plantlets were successfully established in soil.