Mast cell identification and visualization by means of electron microscopy, immunohistochemistry and special staining methods in the course of canine atopic dermatitis

Atopic dermatitis (A D) is a chronic and recurring inflammatory disease with characteristic clinical presentation and significant levels of pruritus. Up to 10% of the canine population is affected atopic dermatitis. Despite many years of scientific research, there are still many of aspects of atopic pathogenesis which require further explanation. The aim of the study was the attempt to present the possibility of displaying mast cell populations in canine atopic dermatitis by using different staining protocols and microscopy techniques. The study was conducted on biopsy material collected from two groups of dogs: atopic and healthy controls. The material was tested by means of light microscopy. Additional study was conducted using TEM Transmission Electron Microscope. A range of staining methods, as well as possible use of different microscopes types in order to identify and evaluate mast cell population in canine atopic skin, are presented and compared. Depending on the technique used it is possible to evaluate numbers, topography and activity of mast cells in canine skin.