Chromosomal heterogeneity of the G-401 rhabdoid tumor cell line: unusual partial 7p trisomy

Abstract (247 words) Rhabdoid tumor is very aggressive and hardly curable pediatric malignancy. It commonly starts in the kidneys but also can occur in brain, liver and other organs. The treatment of this tumor usually involves a combination of surgery, radiation and chemotherapy. Because this tumor is rare, there is still limited experience with a defined standard of care. Cytogenetic analysis is an important routine method to monitor chromosomal aberrations. We have analyzed metaphases of G-401 rhabdoid tumor cell line. In this cell line we have observed metaphases with derivative chromosome 12 arising from partial trisomy 7p. With increasing passage number the numbers of metaphases having this derivative chromosome 12 were found to be higher. In passage number 2 only one metaphase had this pathological chromosome 12. By passage number 10 and passage number 15 about 25% and 95% of this derivative chromosome 12 were found respectively. We were able to subclone G-401 cells by limiting dilutions and successfully separated cells having apparently normal karyotypes from cells having derivative chromosome 12. Using the cell proliferation assay we showed that clones possessing the derivative chromosome 12 grew more rapidly than clones with normal chromosomes. Chromosomal aberrations in chromosome 7 are often have been observed in brain tumors. In this study we describe for the first time a 7p triplication in a rare rhabdoid tumor. Both types of clones described in this study could be used as a preclinical model to study partial chromosome 7 alterations in the development of rhabdoid tumors.