Construction of HV S and 5’Terminal of S Gene Segment Eukarytic Expression Vectors and Transfection into Vero Cells

Abstract：In order tO provide some data for study of T cell epitope．HV S and 5’termirmI of S gene segment were inserted into an eukarytic expression vector pcDNA 3．1／V5一His TOPO．The recombi— nant expression plasmids peDNA3 1-S and pcDNA3．1-S—N were constructed．Vero cells were trans— fected in vitro with them．The transient expresion of HV S and 5’terminal of S gene segment in veto cells w∞ detected by；ndirect immunofluoreseence assay(IFA)．