Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
2016
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-A structure), Thermosynechococcus elongatus photosystem II (<3-A diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-A structure).
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