Platelet activating factor: release fromcolonic mucosa inpatients withulcerative colitis andits effect on colonic secretion

Inflammatory mediators havebeenimplicated inthepathophysiology ofulcerative colitis. Theymay stimulate intestinal secretion andcontribute totheproduction ofdiarrhoea. Platelet activating factor (PAF)mayberesponsible forahighproportion ofthis secretory response. Biopsy specimens frominflamed andquiescent mucosaofpatients withulcerative colitis andnormalhumancolonic mucosawere cultured orco-cultured. Therelease of PAF,prostaglandin E2,andleukotriene D4intotheculture mediumwasmeasured andtheability ofthisculture medium, frominflamed andnormaltissues, to influence secretion inratcolonic mucosa was assessed. PAF was liberated by inflamed tissue. Itsrelease fromquiescent butnotnormaltissue wasstimulated by medium in whichinflamedmucosal biopsy tissues hadbeencultured andby exogenousbradykinin and 5-hydroxytryptamine, butnotbyhistamine. PAF stimulated eicosanoid production. The riseinshortcircuit currentproduced in vitro byinflamed tissue culture medium wasinhibited bythePAFreceptor antagonist(CV6209)(460/o) (32.4(2.9) v 17.5 (1.19) pA.cm-2, p

Keywords:

• Eicosanoid Production
• Prostaglandin E2
• Antagonist
• Tissue culture
• Colitis
• Secretion
• Biopsy
• Endocrinology
• Medicine
• Internal medicine
• Platelet-activating factor
• In vitro

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