Quantitative analysis of biomarkers in hydro-ethanolic extract of Aegle marmelos by High Performance Thin Layer Chromatography (HPTLC)

In the present study the quantitative phytochemical analysis of extract of unripe fruits of Aegle marmelos was carried out with specific biomarkers like gallic acid, quercetin and rutin by high-performance liquid chromatography (HPLC). The detection and quantification of these markers were performed at 200 - 450 nm wavelength with reference to standards in mobile phase Toulene: Ethyl Acetate: Formic Acid: Methanol (3:3:0.3:0.2). The hydro-ethanolic extract of unripe fruits of Aegle marmelos showed peak Rf values which were coinciding with standard Rf value of gallic acid and quercetin. The Rf values for gallic acid and quercetin in sample and reference standard were found comparable. These observations indicated the presence of gallic acid and quercetin in hydro-ethanolic extract of unripe fruits of Aegle marmelos. Results of this study concludes that the presence of gallic acid and quercetine that might be responsible for its antidiarrhoeal, antibacterial, astringent and anti-inflammatory activity in extract.