Electron microscopic visualization of nucleosomal organization in B12 starved and control Euglena chromatin

Abstract Euglena chromatin, spread according to the Dubochet technique, displays the nucleosomal organization with the typical zig-zag conformation following short periods of digestion with micrococcal nuclease. After comparative kinetic experiments on vitamin B12 starved and control Euglena nuclei, a larger percentage of monosomes appears after 2 mm. in the starved chromatin (86%) compared to the control (59%). No difference is found for longer period of incubation.