Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)
2018
: A proteomic platform for global analysis of protein complexes and protein-protein interactions (PPIs) is described. Briefly, after comprehensive physiochemical separation of soluble protein extracts using non-denaturing ion exchange chromatography (IEX), each fraction is subjected to quantitative tandem mass spectrometry analysis.
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