Studies on Safety Assessment of Transgenic Bt Poplar

The purpose is to detect the stability of foreign gene in Bt poplar,the transmission of the transgene to the tree progenies using a protocol on detection Bt gene in tree sample by means of PCR technique.The results show that the gene was stable after seven years in the plantation.Flowers were collected from a male transgenic tree and pollinated in controlled conditions.Progeny seeds were germinated and grown in vitro. Analyses revealed that segregation appears to follow Mendel's rule as 67 out of 120 individual tested harbor the transgene with the expected 1∶1 segregation.;