Self-assembling peptide systems for 2D Tissue Cell Culture

INTRODUCTION: This project exploits octapeptides as building blocks for the selfassembly of fibrous networks that mimic extra cellular matrix (ECM) in living organisms. Scaffolds that mimic the ECM of tissues should be capable of supporting three-dimensional cell culture to promote cell attachment, cell proliferation and differentiation. By varying peptide concentration and type we aim to design and tailor hydrogel stiffness, porosity, viscosity, water content, fiber density and mechanical properties to control cell interactions and subsequent tissue growth. Here we will present our initial work on two peptides that self-assemble to form transparent hydrogels at room temperature; FEFEFKFK and FEFKFEFK.