Metabonomics Study on the Urine in Rat Treated by Water Extract of Zanthoxylumschinifolium Siebet Zucc

Objective:HPLC/MS/MS-based study on the changes of endogenous metabolites of Sprague-Dawley Rats administrated with water extract of Zanthoxylumschinifolium Siebet Zucc. Method:Ten Sprague-Dawley rats were successively administrated with Zanthoxylumschinifolium Siebet Zucc water extract (0. 342 8 g·kg -1 ) for 30 days. Agilent1200 6410 Triple Quadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data were analyzed by OSC-PLS-DA. Result: The difference in metabolic profiles between the control group and the dosed group was well observed by OSC-PLS-DA analysis after administration of 15 days,31 biomarkers were found, such as cytidine, decanoylcarnitine and N-acetylglucosamine 6-phosphate. Conclusion:The administration of Zanthoxylumschinifolium Siebet Zucc water extract could influence concentrations of endogenous matabolites related to anticancer,analgesic and hypolipidemic pharmacological actions. This work can provide basis for further researches on the interpretation of drug property of evodiae.