Multicomponent copolymers of hydrophilic block - Preparation and Application of hybrid silica gel chromatography packing

The present invention discloses present invention discloses a multicomponent hydrophilic block copolymer - Hybrid chromatography on silica gel Preparation and application of the filler, the filler is atom transfer radical polymerization initiator in situ on the particle surface sequentially through successive silica surface polymerization SBMA, GMAG, GMAM other hydrophilic monomers to produce multi-component block copolymers of hydrophilic - hybrid silica filler. The hybrid packing height of surface roughness, thus having a high specific surface area. The use of multi-component block copolymers of hydrophilic - hybrid silica gel solid phase extraction column packing and packed HPLC column successfully rich glycoprotein and standard mouse liver proteins and complex glycopeptide sugar chain sample collection, separation, selective enrichment, separation evident in mouse liver total protein enriched in 1244 identified glycopeptides, glycoproteins corresponding to 577, fully proved its glycosylation analysis of complex proteins in the sample great value.