Identification of a new minor iridoid glycoside in Symplocos glauca by thermospray liquid chromatography-mass spectrometry.

Abstract Iridoid glycosides in Symplocos glauca were identified by thermospray liquid chromatography-mass spectrometry. The conditions for optimization of the separation of iridoid glycosides were investigated. The mass spectra obtained gave qualitative information concerning both the aglycone and sugar moieties and also the molecular weights. Methanol extracts from the leaves of S. glauca were analysed for iridoid glycosides. Not only major but also minor components could be separated and identified from the mass spectra and mass chromatograms. A new minor component in S. glauca was tentatively identified as 6-dihydroverbenalin.