Preparation method for acetylated chitotriose

The present invention pertains to marine chemical engineering, particularly to a chitotriosidase N- acetylated and N, N'- preparation and separation and purification method of diacetylated of chitotriosidase. Specifically, first preparing the whole chitotriosidase deacetylated, deacetylated after full chitotriosidase was dissolved in methanol / water solution was added at room temperature a quantity of acetic anhydride were acetylation to give N- acetylated mixture chitotriosidase , then the mixture with an ion exchange CM Sephadex C-25 column chromatography or CM Sepharose Fast flow 0-1M NaCl solution concentration to be separated and purified at pH 4-6, a flow rate of 2-5mLmin-1 of eluate was collected the eluted fractions were desalted and concentrated after the extraction of activated carbon, i.e., to give N- acetylated chitotriosidase and N, N'- diacetyl chitotriosidase.