Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique
2007
Control of membrane voltage and membrane current measurements are of strong interest for the study of numerous aspects of skeletal muscle physiology and pathophysiology. The silicone-clamp technique makes use of a conventional patch-clamp apparatus to achieve wholecell voltage clamp of a restricted portion of a fully differentiated adult skeletal muscle fiber. The major part of an isolated muscle fiber is insulated from the extracellular medium with silicone grease, and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. This method represents an alternative to the traditional vaseline-gap isolation and two or three microelectrode voltage-clamp techniques. This chapter reviews the main benefits of the silicone-clamp technique and provides detailed insights into its practical implementation.
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